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Wild type green fluorescent protein (wt-GFP) and the variant S65T/H148D each exhibit two absorption bands, A and B, which are associated with the protonated and deprotonated chromophores, respectively...
A random library of single amino acid mutants of myoglobin was generated using a highly efficient, single-base-misincorporation random mutagenesis method to discover new ligand-binding pathways in myo...
Photosynthetically active chimeric reaction centers which utilize genetic information from both Rhodobacter capsulatus and Rb. sphaeroides puf operons were isolated using a novel method termed chimeri...
Fatty acid elongation is the extension of de novo synthesized fatty acids through a series of four reactions analogous to those of fatty acid synthase. ELOs catalyze the first reaction in the elongati...
Interaction of ribosomal protein L1 and the 23S rRNA plays an important role in both the structure and biological activity of the Escherichia coli ribosome. We have minimized the binding site for prot...
Candida albicans and Candida glabrata are opportunistic human pathogens that are the leading cause of fungal infections, which are increasingly becoming the leading cause of sepsis in immunosuppressed...
北京师范大学分子生物学课件CHAPTER 6 Mutations, Mutagenesis and DNA Repair。
以携带pUB110质粒的枯草芽孢杆菌BR151菌株为出发菌株,利用亚硝基胍作诱变剂,直接在LB平板上进行诱变。从2949个菌落中筛选出一个转化频率低于出发菌株2~3个数量级的突变株,并对其营养缺陷型、UV的敏感性及Km抗性和质粒进行了检测,确证其转化能力降低为自然感受态缺陷而非营养缺陷型的改变或重组缺陷所致。

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