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Expression, Purification, and Characteristic of Tibetan Sheep Breast Lysozyme Using Pichia pastoris Expression System
Pichia pastoris Purification Secretory Expression Tibetan Sheep
2016/5/16
A lysozyme gene from breast of Tibetan sheep was successfully expressed by secretion using a-factor signal sequence in the methylotrophic yeast, Pichia pastoris GS115. An expression yield and specific...
Fleece Phenotype Influences Susceptibility to Cortisol-induced Follicle Shutdown in Merino Sheep
Merino Strongwool Finewool Cortisol Injection Follicle Shutdown
2016/4/26
This experiment was conducted to determine the extent to which susceptibility to cortisol-induced follicle shutdown is influenced by fleece phentotype. Twenty Finewool (10 sheep low fibre diameter, lo...
Comparison of the [2H5]Phenylalanine Model with the [1-13C]Leucine Method to Determine Whole Body Protein Synthesis and Degradation in Sheep Fed at Two Levels
Isotope Dilution Method Stable Isotope Dietary Intake Protein Synthesis Protein Degradation Sheep
2016/4/26
The [2H5]phenylalanine model was compared with the [1-13C]leucine method to determine whole body protein synthesis (WBPS) and degradation (WBPD) in sheep fed at two levels. The animals were fed either...
Molecular Characterisation of Nilagiri Sheep (Ovis aries) of South India Based on Microsatellites
Genetic Characterisation Nilagiri Sheep Microsatellites PIC Inbreeding Estimates
2016/4/25
Genetic variation in Nilagiri sheep, the only apparel wool breed in South India was studied using 25 FAO recommended ovine-specific microsatellite markers. The number of observed alleles ranged from 3...
Diagnosis of Benzimidazole Resistance in Haemonchus contortus of Sheep by Allele Specific PCR
Haemonchus contortus Benzimidazole Resistance beta Tubulin AS-PCR
2016/4/25
The study was conducted on 162 adult male Haemonchus contortus of sheep collected from Avikanagar, Jaipur and Bikaner regions to diagnose the benzimidazole (BZ) resistance in H. contortus. The BZ resi...
Change in Nitrogen Fractions and Ruminal Nitrogen Degradability of Orchardgrass Ensiled at Various Moisture Contents and the Subsequent Effects on Nitrogen Utilization by Sheep
Orchardgrass Moisture Contents Ensiling Nitrogen Fractions Ruminal Degradability Nitrogen Utilization
2016/4/20
The effect of various moisture contents of fresh forage on the change in nitrogen (N) fractions, in vitro ruminal N degradability, and the subsequent N utilization of silage in sheep were evaluated. O...
Evaluation of Fishmeal Supplement with Net Nitrogen Flux by the Portal-drained Viscera and the Liver in Mature Sheep
Fishmeal Absorption Amino Acid Ammonia Urea Sheep
2016/4/20
The objective of this study was to evaluate the net flux response of nitrogen compounds (alpha-amino N, ammonia N, urea N, essential amino acids) across the portal-drained viscera (PDV), liver and tot...
Effect of Intraruminal Sucrose Infusion on Volatile Fatty Acid Production and Microbial Protein Synthesis in Sheep
Branched-chain Fatty Acid Grass Silage Microbial Protein Synthesis Sucrose
2016/4/19
Effects of sucrose supplement on the pattern of VFA production and microbial protein synthesis in the rumen were examined in sheep consuming basal diet of grass silage (2.5 kg fresh wt/d) that was pro...
Change in Nitrogen Fractions and Ruminal Nitrogen Degradability of Orchardgrass and Alfalfa during the Ensiling Process and the Subsequent Effects on Nitrogen Utilization by Sheep
Orchardgrass Alfalfa Ensiling Nitrogen Fractions Ruminal Degradability Nitrogen Utilization
2016/4/18
In order to determine the extent of change in nitrogen fractions and in vitro ruminal degradability of forage protein during ensilage and the influence on nitrogen utilization by sheep, orchardgrass (...
Effects of Combination of Nitrate with β1-4 Galacto-oligosaccharides and Yeast (Candida kefyr) on Methane Emission from Sheep
Rumen Methanogenesis Nitrate 1-4 Galacto-oligosaccharide Candida kefyr Methaemoglobin
2016/4/14
The objective of the present study was to determine whether 1-4 galacto-oligosaccharides (GOS) and Candida kefyr combined with nitrate as manipulators could suppress rumen methanogenesis without nitra...
Effect of Feeding Chemically Treated Mustard (Brassica campestris) Straw on Rumen Fibre Degrading Enzymes in Sheep
Chemical Treatments Mustard Straw Rumen Fibre Degrading Enzyme Sheep
2016/4/14
The aim of the experiment was to study the changes in the activities of various rumen fibre degrading enzymes due to the feeding of chemically treated mustard (Brassica campestris) straw in sheep. Mus...
Effects of Concentrate to Roughage Ratio on the Formation of cis9, trans11-CLA and trans11-Octadecenoic Acid in Rumen Fluid and Plasma of Sheep When fed High Oleic or High Linoleic Acid Oils
Concentrate to Roughage Ratio Oil Source Cis9 trans11-C18:2 Trans11-C18:1 Sheep
2016/4/14
A metabolism trial with four ruminally fistulated sheep was conducted in a 4횞4 Latin square design to examine the effect of concentrate to roughage ratio (70:30 vs. 85:15) and oil source (soybe...
A Study on the Optimal Amino Acid Pattern at the Proximal Duodenum in Growing Sheep
Amino Acids Duodenum Growing Sheep
2016/4/8
Nine crossbred castrated lambs fitted with rumen and duodenum cannula and fed a diet of hay and concentrate formulated with ground corn and soybean meal as main ingredients were used to assess the duo...