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Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants
Agrobacterium tumefaciens genetic engineering GUS activity Picea abies Karst
2015/3/27
In conifers and other plants with long reproductive cycles, transformed embryogenic tissues can serve as a convenient source of plant material for the testing of insecticidal or fungicidal transgene e...
Effects of different sources of iron, hormones and Agrobacterium tumefaciens on the chlorophyll and iron concentration in the leaves of peach trees
acidic compounds hormones iron chlorosis peach cultivars rootstocks
2015/1/22
The aim of this study was to investigate the effect of different sources of iron, hormones and Agrobacterium tumefaciens in the chlorophyll and iron concentration of the peach cultivars Katerina and F...
An improved Agrobacterium tumefaciens mediated transformation of Artemisia annua L. by using stem internodes as explants
anti-malaria artemisinin stem internode traditional Chinese medicine transgene
2014/2/24
Transformation of Artemisia annua, which produces the sesquiterpenoid endoperoxide artemisinin widely used for the treatment of malaria, has been hampered by the low efficiency of adventitious shoot a...
The effect of different antibiotics on the elimination of Agrobacterium and high frequency Agrobacterium-mediated transformation of indica rice (Oryza sativa L.)
Agrobacterium-mediated transformation Agrobacterium elimination gus reporter gene hygromycin phosphotransferase selectable marker gene (hpt) rice
2014/2/25
We report here the suitable explant with high efficiency of transformation and the positive effects of timentin over other antibiotics like carbenicillin and cefotaxime on the elimination of Agrobacte...
Agrobacterium Tumefaciens Nitrogen Fixation System Expresses in Sinorhizobium meliloti (=Rhizobium meliloti)
Acetylene reduction dry weight root nodules Alfalfa Sinorhizobium meliloti Rhizobium meliloti Agrobacterium tumefaciens
2010/11/16
Kanvinde and Sastry (Appl. and Environ. Microbiol. 56:2087-2092, 1990) had previously
demonstrated that A. tumefaciens has all the physiological characters of a diazotroph. Genetic evidence indicates...
Rooting Induction of Soft Wood and Almond Micro-cutting Using Agrobacterium Rhizogenes
rooting induction almond micro cutting Agrobacterium rhizogenes
2010/11/15
The present work was carried out during the period between 1999 till 2003 in the laboratories
of Agriculture Development System (ADS). Cairo, Egypt. This study aimed to investigate rooting induction ...
Agrobacterium-Mediated Transformation in Liliaceous Ornamental Plants
Faculty of Agriculture,Niigata University,Japan
2009/9/10
Studies on Agrobacterium-mediated transformation in 3 Liliaceous ornamental plants, Lilium formosanum, Agapanthus praecox ssp. orientalis and Muscari armeniacum, were described. Three different strain...
Antibiotics for Agrobacterium Elimination in Apple Transformation
cefotaxime Malus × domestica Borkh. meropenem shoot regeneration
2010/11/23
We investigated several antibiotics including carbenicillin (CBPC), clavulanic acid/amoxicillin (CVA/AMPC), cefotaxime (CTX), meropenem (MEPM), vancomycin (VCM) and doxycycline (DOXY) for Agrobacteriu...
Tissue Culture-independent In Planta Transformation Strategy：an Agrobacterium tumefaciens-Mediated Gene Transfer Method to Overcome Recalcitrance in Cotton (Gossypium hirsutum L.)
Planta Transformation Strategy Agrobacterium tumefaciens-Mediated Gene Transfer Method Cotton
2009/4/30
The major constraint in cotton improvement has been the recalcitrance of cotton varieties to tissue culture. Alternate methods that avoid/minimize tissue culture would be beneficial for the improvemen...
Agrobacterium tumefaciens mediated transformation and regeneration of ginger（Zingiber officinale Rosc）
Genetic transformation b-glucuronidase GUS expression
2009/2/19
The absence of seed set in ginger makes conventional breeding methods inapplicable warranting genetic modification through biotechnological means. Agrobacterium tumefaciens strain EHA105/p35SGUSIn...
Detection of Crown Gal Agent（Agrobacterium tumefaciens）in Grapevine Propagating Material
Crown Gal Agent Grapevine Propagating Material
2009/1/23
Polyclonal antiserum produced against Agrobacterium tumefaciens (biovar 3) reacted in immunofluorescent test with biovar 3 strains of A.tumefaciens,causal agent of crown gal, from Central Anatolia. T...
Effect of Indole-3-Butyric Acid and Different Strains of Agrobacterium rubi on Adventive Root Formation from Softwood and Semi-Hardwood Wild Sour Cherry Cuttings
Sour cherry softwood and semi-hardwood cutting Agrobacterium rubi IBA adventive rooting
2009/1/13
This study was conducted to evaluate the effects of a range of Indole-3-butyric acid (IBA) concentrations (250, 500 and 750 mg 1-1) alone and in combination with three strains of Agrobacterium rubi (A...
Effect of Phytosulfokine-α on Agrobacterium-Mediated Transformation in Rice
phytosulfokine rice Agrobacterium-mediated transformation
2009/1/7
Phytosulfokine-α(PSK-α), a biologically active peptide acting as a growth factor, plays a key role in cellular differentiation and proliferation. To test if PSK-αhas some influence on agrobacterium-me...
Linkage Analysis of Transgenes Inserted into Cotton via Agrobacterium tumefaciens Transformation
Linkage Analysis Transgenes Cotton Agrobacterium tumefaciens Transformation
2008/12/15
The location of transgenes inserted into a genome are important in genetic studies and breeding programs. We conducted linkage analysis between 2,4-D resistant transgenes and 14 morphological marker g...
Development of Transgenic Tall Fescue Plants from Mature Seed-derived Callus via Agrobacterium-mediated Transformation
Tall Fescue Agrobacterium Transgenic Plants Callus Forage
2016/4/18
We have achieved efficient transformation system for forage-type tall fescue plants by Agrobacterium tumefaciens. Mature seed-derived embryogenic calli were infected and co-cultivated with each of thr...