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Decrease in the number of human Ap and Ad spermatogonia and in the Ap/ Ad ratio with advancing age. New data on the spermatogonial stem cell
human testis spermatogonia aging
2009/2/23
The numbers of Ap and Ad spermatogonia per unit section of the testis were calculated in autopsy specimens from young adults and elderly men without testicular pathology. The number of Ap spermatogo...
Hormone treatment after irradiation stimulates recovery of rat spermatogenesis from surviving spermatogonia
Testis stem cells GnRH-agonist LHRH testosteron
2009/2/17
The possibility of stimulating the recovery of spermatogenesis after irradiation using hormone treatment was tested in LBNF, rats. At 10 weeks after irradiation with 3.5 Gy, the percentage of tubu...
Isolation of highly purified type A spermatogonia from prepubertal rat testis
Type A spermatogonia cell separation c-kit cell culture
2009/2/17
We have developed a new method that allows isolation of highly purified type A spermatogonia from prepubertal rats. The procedure is based on the maximal release of spermatogonia from the seminifero...
Ultrastructural observations of spermatogenesis in mice resulting from transplantation of mouse spermatogonia
Testis mouse spermatogenesis electron microscopy
2009/2/16
The objective of the present study was to provide a morphological characterization of spermatogenesis following germ cell transplantation into the seminiferous tubular lumen of another mouse. The re...
Testicular composition, number of A spermatogonia, germ cell ratios, and number of spermatids in three different breeds of boars
Breed boar testis composition
2009/2/16
Seminal quality, including the number of spermatozoa in the ejaculate, varies among breeds of boars. Variation in seminal quality may be explained by breed differences in testicular size and compo...
Hormonal protection from cyclophosphamide-induced inactivation of rat stem spermatogonia
Hormonal protection cyclophosphamide-induced inactivation rat stem spermatogonia
2009/2/13
Studies of protection of testicular function from cyclophosphamide with hormonal pretreatment have been limited by the lack of a convenient model for cyclophosphamide-induced inactivation of stem ...
2,5-hexanedione exposure in the rat results in long-term testicular atrophy despite the presence of residual spermatogonia
Germ cell repopulation Sertoli cell toxicity spermatogonia
2009/2/7
Charles River CD rats (approximate weight, 208 g) were exposed to 1.0% 2,5-hexanedione (2,5-HD) in drinking water for 5 weeks. Rats were killed 27, 60, and 75 weeks after exposure to evaluate the re...
Expression of Notch pathway components in spermatogonia and Sertoli cells of neonatal mice
Cell fate delta jagged presenilin spermatogenesis
2008/12/29
Members of the Notch gene family have been shown to play an important role in the control of cell fate in many developmental systems. We hypothesized that the fate of the male germ line stem cells m...
Immunomagnetic isolation and long-term culture of mouse type A spermatogonia
Germ cells c-kit Ep-CAM magnetic beads Sertoli
2008/12/29
In the mammalian testis, type A spermatogonia proliferate and differentiate into sperm under the tight control of both endocrine and paracrine factors. In order to study the complex process of sperm...
Development of a Cryopreservation Protocol for Type A Spermatogonia
Freezing spermatogonial stem cells transplantation
2008/12/5
The aim of this study was to develop a cryopreservation protocol for type A spermatogonia. Testes from 5- to 7-month-old calves were collected, and type A spermatogonia were isolated using two-step ...