搜索结果: 1-11 共查到“基础医学 FISH”相关记录11条 . 查询时间(0.093 秒)
Prenatal exposure to methyl mercury from fish consumption and polyunsaturated fatty acids: associations with child development at 20 mo of age in an observational study in the Republic of Seychelles
child development maternal fish consumption methyl mercury polyunsaturated fatty acids docosahexaenoic acid arachidonic acid n–6:n–3
2018/12/24
Background: Fish is a rich source of n–3 polyunsaturated fatty acids (PUFAs) but also contains the neurotoxicant methyl mercury (MeHg). PUFAs may modify the relation between prenatal MeHg exposure and...
FISH Analysis for del6q21 and del17p13 in B-cell Chronic Lymphocytic Leukemia in Iranians
Leukemia Lymphocytic Chronic B-Cell Cytogenetic Aberrations Biological Markers
2015/9/29
Background: B-cell chronic lymphocytic leukemia (B-CLL) is the most common leukemia in the Western world. Major progress has been made in assessing typical chromosomal abnormalities and recognition of...
The Effect of Fish Oil on Liver and Plasma MDA and Antioxidant Status of Rats
Fish Oil Antioxidant Erythrocyte GSH levels
2009/7/2
The question how fish oil (FO) and soybean oil (SO) change the state of antioxidant and the lipid peroxidation in liver and plasma of rats and the protective role of vitamin E were investigated. Six-w...
Chromosomal Aberrations in Radiation Waste Repository Workers Detected by Fish Painting and Giemsa Staining
Occupational radiation exposure Human lymphocytes Chromosomal aberrations Fluorescent in situ hybridization
2009/6/23
We aimed to assess chromosomal aberration frequency by means of FISH chromosome painting and conventional Giemsa staining in peripheral blood lymphocytes of workers at a nuclear waste repository. The ...
FISH技术评价昆明山海棠在小鼠骨髓细胞中的非整倍体诱发效应
荧光原位杂交 昆明山海棠 小鼠骨髓细胞 非整倍体
2008/10/17
目的: 研究以荧光原位杂交(fluorescence in situ hybridization , FISH) 技术评价了中草药昆明山海棠根部水抽提物[ Tripterygium hypoglaucum ( Level) Hutch , THH]在小鼠骨髓细胞中的特异染色体不分离效应。方法:以受试物THH 腹腔注射昆明种雄性小白鼠,24 h 后取骨髓细胞常规制片,以bio-16-dUTP 标记的...
背景与目的:探索多色荧光原位杂交(Multicolor fluorescence in situ hybridization, M-FISH)技术分析急慢性电离辐射照射诱发的小鼠染色体畸变的差异。 材料与方法: 建立用小鼠1、2和4号染色体端粒和着丝粒特异性人工细菌染色体(Bacterial Artificial Chromosome,BAC) 的M-FISH方法,分析受到急慢性60Co ...
应用FISH技术对我国早期从事医用X线工作者的细胞遗传学评价
荧光原位杂交 X 线工作者 染色体畸变 淋巴细胞
2008/8/28
目的:对受到长期、低剂量率照射的我国早期从事医用X 线工作者辐射损伤的远期效应进行细胞遗传学观察和评价。方法:用4 号和7 号全染色体探针和荧光原位杂交技术,分析25 名早期X 线工作者和10 名对照者外周血淋巴细胞染色体畸
变,并用Giemsa 染色验证。结果:直观地表现出正常靶染色体和多种畸变。X 线工作者的易位占其染色体总畸变的84 %;其他各种畸变率分别为插入( Ins) 1. 9 %、...
荧光原位杂交(FISH) 检测人精子染色体非整倍体率方法的研究
荧光原位杂交 人精子染色体 非整倍体率
2008/8/28
本研究建立了荧光原位杂交(FISH) 方法,并测定了十名正常健康男子精子X 染色体非整倍体率。取少量精液经洗后制片,用二梳苏糖醇(DTT) 和二碘水杨酸锂(L IS) 处理,使精子头部紧密的染色质去凝集,然后与生物素标记的α- 卫星X 染色体特异DNA 探针(DXZ1) 作原位杂交;用Cy3 - 链亲和素检测杂交信号。结果在荧光显微镜下可清楚见到带有红色杂交点信号的X 精子。杂交率平均为48.62...
用荧光原位杂交 ( FISH) 检测接触二硫化碳 CS2 工人精子 X染色体非整倍体率的初研究。
用染色体FISH方法检测暴露于不同环境诱变因素居民的吸烟效应
染色体易位 天然辐射 环境诱变剂 吸烟
2008/8/27
背景与目的: 研究包括天然辐射等不同环境诱变因素地区居民的吸烟效应。 材料与方法: 外周血采自10名在北京居住超过40年的吸烟居民,通过使用便携式个人剂量计随身测量24 h所得的剂量估算累积辐射剂量,通过荧光原位杂交(FISH)方法对北京吸烟居民的外周血淋巴细胞进行染色体易位的分析,其结果与10名广东高天然本底辐射(高本底)地区吸烟居民,7名对照地区吸烟居民,以及北京、高本底地区和对照地区的20、...