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BIOCHEMICAL APPLICATIONS OF DSRED-MONOMER UTILIZING FLUORESCENCE AND METAL-BINDING AFFINITY
fluorescent protein labeling non-natural mutagenesis ligand fishing protein-protein interactions fluorescence quenching metal-binding affinity red fluorescent protein
2015/5/21
The discovery and isolation of naturally occurring fluorescent proteins, FPs, have provided much needed tools for molecular and cellular level studies. Specifically the cloning of green fluorescent pr...
Screening of Aflatoxin-producing mould isolates based on fluorescence production on a specific medium under ultraviolet light
Aflatoxin Fluorescence Pistachio nut Aspergillus Specific medium
2009/12/28
Aflatoxin contamination of food and feedstuffs is a serious economic and health hazard. Over a period of two years, 35 samples of raw pistachio nuts collected from different parts of Iran were analyse...
Comparison of Conventional Cytogenetic and Fluorescence In Situ Hybridization Results of Prenatal Aneuploidy Screening
Aneuploidy prenatal diagnostic tests FISH conventional cytogenetics
2009/6/17
Background and Aim: Prenatal diagnosis of chromosome abnormalities is usually performed on amniotic fluid samples. Aneuploidy screening by fluorescence in situ hybridization (FISH), although not an al...
用间期荧光原位杂交检测卵巢癌细胞中X染色体数目的异常The Application of Interphase Fluorescence in situ Hybridization to the Diagnosis of X Chromosomal Count Abnormality in Ovarian Carcinoma Cell
荧光原位杂交 卵巢癌 X染色体数目异常 DNA特异性探针
2008/1/10
摘要为了探讨用荧光原位杂交技术(fluorescence in situ hybridization, FISH)检测卵巢癌细胞中性染色体拷贝数目异常的实验方法及其应用价值,收集18例新鲜卵巢癌组织标本,以Biotin标记的X染色体α-卫星DNA(pBamX7)探针与经处理的标本进行卵巢癌细胞核的原位杂交,分别用Avidin-FITC和Anti-avidin进行信号的检测与放大,PI复染。于Oly...
Development of Fluorescence-linked Immunosorbent Assay for High Throughput Screening of Interferon-γ
enzyme-linked immunosorbent assay fluorescence-linked immunosorbent assay fluorometric microvolume assay technology high-throughput screening interferon-gamma
2010/11/18
We developed a new system for measuring hIFN-γ using Allophycocyanine (APC) fluorescent protein and compared it with the previous method using Cy5.5. The proposed FLISA had a smaller coefficient of va...