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Use of peanut agglutinin to assess the acrosomal status and the zona pellucida-induced acrosome reaction in stallion spermatozoa
FITC-PNA hemizona acrosome reaction
2009/2/17
Peanut agglutinin (PNA) was used to assess the sperm acrosomal status and the acrosome reaction during gamete interaction in the equine species. PNA exclusively binds to the outer acrosomal membra...
The fix vital stain method. Simultaneous determination of viability and acrosomal status of bovine spermatozoa
Acrosomal evaluation vital staining Hoechst bisbenzimide
2009/2/7
A rapid, accurate and precise method for simultaneous determination of acrosomal status and viability of bull spermatozoa is introduced and evaluated. The method involves fixation of semen with glut...
Effect of sperm-associated antibodies on the acrosomal status of human sperm
Antisperm antibodies acrosome reaction infertility spermatozoa
2009/2/6
The acrosomal status of human sperm was assessed by the specific binding of Pisum sativum lectin to the acrosomal matrix. Immunoglobulin G (IgG) fractions of plasmas that were positive for IgG antis...
Assessment of the acrosomal status of ram spermatozoa by RCA lectin-binding and partition in an aqueous two-phase system
acrosomal status ram spermatozoa RCA lectin-binding partition aqueous two-phase system
2008/12/25
The acrosome reaction is an important marker for sperm function. Because different laboratory techniques may be used to detect this exocytotic process, the objective of this study was to investiga...