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水稻硝酸盐转运蛋白基因OsNPF7.9在氮素积累和转运中的功能研究
水稻 氮素 OsNPF7.9 硝酸盐转运蛋白
2018/1/29
【目的】植物NPF家族成员具有转运硝酸盐、小肽等的功能。对水稻OsNPF7.9基因的功能研究能够为水稻氮素高效利用的分子机制提供理论基础。【方法】利用不同的生物软件对OsNPF7.9蛋白的生物学信息进行了预测分析;用OsNPF7.9基因启动子融合GUS报告基因的转基因水稻进行OsNPF7.9的组织定位观察;利用半定量RT-PCR和pOsNPF7.9::GUS转基因水稻分析OsNPF7.9受氮素调控...
坛紫菜壳孢子萌发的第一次和第二次分裂为减数分裂,结束后形成了一个基因型不同的四分体,随后由这个四分体发育成一个基因型嵌合的叶状体。由坛紫菜与长紫菜种间杂交所产生的杂合丝状体成熟后释放出壳孢子,后者在分裂初期出现了大量的细胞崩溃死亡,通过对单个的壳孢子萌发体进行定点跟踪观察后发现,细胞崩溃死亡不仅会发生在减数分裂的直接产物即处于二细胞期、三细胞期、四细胞期的萌发体,当细胞数大于4个的萌发体在进行有丝...
The influence of genetic susceptibility and calcium plus vitamin D supplementation on fracture risk
fracture bone mineral density genetic risk score calcium vitamin D postmenopausal women
2018/11/13
Background: Fracture is a complex trait, affected by both genetic and environmental factors. A meta-analysis of genome-wide association studies (GWASs) identified multiple bone mineral density (BMD) a...
探讨巨噬细胞在血管紧张素Ⅱ(AngⅡ)高血压引起内皮细胞功能障碍和心肌肥厚中的作用及机制。 方法 C57BL/6小鼠随机分为正常+PBS组、正常+氯膦酸二钠脂质体(CL)组、AngⅡ+PBS组和AngⅡ+CL组。通过尾静脉注射PBS或CL, 采用植入式胶囊渗透泵灌注AngⅡ。采用小鼠尾套法测量小鼠治疗前、治疗第7天、第14天收缩压;通过HE染色法观察小鼠心肌细胞肥厚程度;血管环张力实验检测血管内皮...
干旱胁迫对甘蔗4个生理指标遗传变异的影响
甘蔗 干旱胁迫 生理指标 遗传变异
2017/11/24
研究干旱胁迫对气孔导度(Gs)、PSⅡ原初光能转化效率(Fv/Fm)、叶片伸长速率(leaf elongation,LE)和叶片相对含水量(relative water content,RWC)4个甘蔗生理指标遗传变异的影响,为其在甘蔗育种程序早期阶段的应用提供参考。【方法】采用裂区设计,以自然干旱和人工灌溉为主区,不同甘蔗基因型为副区,在云南省红河州开远市和玉溪市元江县2个试验点先后对22个和1...
为了解淇河鲫(Carassius auratus var. Qihe)甘露糖受体(Mannose Receptor, MR)的结构特点及其在抗感染免疫反应中的作用,本研究通过同源克隆和RACE技术,获得了淇河鲫甘露糖受体(CaMR)全长cDNA,并采用实时荧光定量PCR(qPCR)技术研究了嗜水气单胞菌感染对CaMR组织表达的影响。结果表明,CaMR cDNA全长4473 bp,5’非编码区81 ...
Effects of supplementation with nondigestible carbohydrates on fecal calprotectin and on epigenetic regulation of SFRP1 expression in the large-bowel mucosa of healthy individuals
nondigestible carbohydrates colorectal cancer calprotectin Wnt signaling epigenetics
2018/11/12
Background: Hyperactive Wnt signaling is frequently observed in colorectal cancer. Higher intakes of dietary fiber [nondigestible carbohydrates (NDCs)] and the fermentation product butyrate are protec...
目的:构建靶向1型1-磷酸鞘氨醇受体(sphingosine 1-phosphate receptor-1, S1P1)基因的小干扰RNA(small interfering RNA,siRNA)慢病毒表达载体,感染干燥综合征细胞模型——人涎腺导管上皮细胞(human salivary gland cells,HSG),探讨S1P1的siRNA治疗干燥综合征的可能性,为临床靶标治疗提供广阔的思路。...
混合多目标遗传算法求解地下水污染修复管理模型
混合多目标算法 NSGAII 局部搜索 地下水污染修复
2016/11/1
为了提高多目标遗传算法Pareto解的局部最优性,本文将快速非支配遗传算法(NSGAII)与一种迭代式的局部搜索算法(Hill Climber with Step,HCS)相结合,开发了一种新的混合多目标遗传算法NSGAII-HCS.利用CONV1和ZDT6两个经典的多目标优化函数对NSGAII-HCS的性能进行测试,与传统的多目标算法NSGAII相比,CONV1得到的Pareto锋面与真实Par...
Heritability of high sugar consumption through drinks and the genetic correlation with substance use
addiction genetics substance use sugar consumption twin study
2018/12/14
Background: High sugar consumption contributes to the rising prevalence of obesity. Sugar can have rewarding effects that are similar to, but less strong than, the effects of addictive substances. Peo...
Heritability of high sugar consumption through drinks and the genetic correlation with substance use
addiction genetics substance use sugar consumption twin study
2018/12/18
Background: High sugar consumption contributes to the rising prevalence of obesity. Sugar can have rewarding effects that are similar to, but less strong than, the effects of addictive substances. Peo...
Interactions between genetic variants associated with adiposity traits and soft drinks in relation to longitudinal changes in body weight and waist circumference
adiposity traits genetic variants interactions obesity soft drinks
2018/12/14
Background: Intake of sugar-sweetened beverages is associated with obesity, and this association may be modified by a genetic predisposition to obesity.
Sugar-sweetened beverage consumption and genetic predisposition to obesity in 2 Swedish cohorts
sugar-sweetened beverage genetic risk score BMI gene-lifestyle interaction Sweden
2018/12/14
Background: The consumption of sugar-sweetened beverages (SSBs), which has increased substantially during the last decades, has been associated with obesity and weight gain.
How much do we know about the heritability of BMI?
heritability of BMI obesity heritable contribution of genetics
2018/12/3
Is obesity heritable? Like father, like son; like mother, like daughter—scientists have no doubt about it. But how great is the contribution of genetics compared with environmental influence? Many twi...
Is obesity heritable? Like father, like son; like mother, like daughter—scientists have no doubt about it. But how great is the contribution of genetics compared with environmental influence?