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鸡肉中氟苯尼考ELISA检测方法的建立及应用
氟苯尼考 残留检测 间接竞争ELISA
2009/8/31
【目的】制备抗氟苯尼考（FFC）的高亲和力特异性抗体，建立检测氟苯尼考的间接竞争ELISA新方法。【方法】氟苯尼考分别与牛血清白蛋白（BSA）和人血清白蛋白（HSA）经混合酸酐法偶联，得到免疫抗原和包被抗原，其偶联比分别为14.4和14.7。用免疫原FFC-HS-BSA免疫新西兰大白兔获得了效价较高的特异性抗体。建立并优化了间接竞争ELISA检测方法。【结果】抗血清效价为1﹕102 400。最佳包...
【目的】克隆猪囊尾蚴AgB基因并在体外细胞中表达。【方法】采用 RT-PCR法分别扩增AgB基因的上半段和下半段序列。采用重叠延伸PCR法（splicing overlap extension PCR method，SOE-PCR）把具有35个相同碱基的上下半段扩增为全长基因，并构建到真核表达载体pVAX1，将酶切、PCR、测序鉴定的阳性质粒经脂质体转染 BHK-21细胞。通过SDS-PAGE、W...
Identification of Transferred Chicken Germ Cells in Quail Gonad and Semen by Amplification of Chicken-Specific PCR Products
microsatellite DNA chicken PCR primordial germ cells quail
2009/8/3
A polymerase chain reaction (PCR) primer set for a chicken microsatellite locus on the Z chromosome, LEI0171 (GenBank Accession No.X85538), amplified a 363bp fragment of genomic DNA in chickens (Gallu...
Effects of Dietary Adenine on Growth,Feed Intake,Purine Excretion and Tissue Purine Concentrations in Young Chickens
dietary adenine adverse effects growth retardation adenine metabolites young chickens
2009/7/31
It was examined whether dietary adenine has adverse effects on growth and feed intake in 2-month-old chickens as reported previously in younger chickens. The male chickens were fed ad libitum on contr...
Osteoclast-Like Cell Formation in Medullary Bone Marrow Cell Culture of Laying Hens
hen medullary bone osteoclast egg-laying cycle culture
2009/7/31
Marrow cells were isolated from medullary bone of hens at two opposing phases, the bone formative and resorptive phases, of the egg-laying cycle. These cells were cultured for up to 14 days on cover s...
The Effect of L-Ascorbic Acid-2-Phosphate Magnesium on Chicks Injected a Radical Initiator
chick L-ascorbic acid-2-phosphate magnesium radical initiator oxidative stress
2009/7/31
Effect of L-ascorbic acid phosphate magnesium (APM) on chicks injected radical initiator, 2, 2′-azobis(2-amidinopropane) dihydrochloride (AAPH), was examined. Chicks were fed APM as ascorbate resourc...
The present experiment was designed to investigate a possibility of the in vitro cultured somatic cells to produce the germ line interspecific chimera in bird. The frozen-thawed fibroblast-like cells ...
The Effects of Intrauterine Injection with Prostaglandin F2α,Arachidonic Acid and Phosphate Solution on the Eggshell Formation of Japanese Quail Hen
eggshell formation prostaglandin arachidonic acid phosphate quail
2009/7/31
Inhibitory effects of prostaglandin F2α, arachidonic acid and phosphate solution on the eggshell formation were investigated in Japanese quail. All injections were performed intrauterinely 8h before t...
Changes of Lysosomal Hydrolase Activity in the Anterior Pituitary of Hens during Induced Molting
chicken induced molting lysosomal hydrolase activity acid phosphatase
2009/7/31
The goal of this study was to determine whether lysosomal hydrolase activity changes during induced molting in the hen pituitary. White Leghorn aged laying hens were subjected to induced molting by fe...
Effects of Exogenous Estrogen on the Differentiation and Development of the Right Oviduct in Female Chickens
estrogen right oviduct sex differentiation chicken
2009/7/31
On day 5 of incubation, 0.64mg of estradiol benzoate (EB) was administered to fertilized eggs to investigate its effects on the differentiation and development of the oviduct in female chickens.
The ...
Chemical Composition and Metabolizable Energy of Mustard Meal
mustard meal plant protein chemical composition metabolizable energy poultry
2009/7/31
Mustard meal (MM), which is a residue of essential oil extraction, had high moisture content (77%). It took 3 days to dry under the sun or 48 hours to dry in an electrical oven at 65°C or 8 hours in a...
Stimulation of ZPC Production by Follicle-Stimulating Hormone in the Granulosa Cells of Japanese Quail (Coturnix Japonica)
Coturnix japonica follicle FSH granulosa cells ovary perivitelline membrane quail ZPC
2009/7/31
Avian perivitelline membrane, an investment homologous to the zona pellucida of mammalian oocytes, is composed of two glycoproteins. Previous studies have indicated that one of the components, a glyco...
A Fermentation Method to Dry and Convert Shochu Distillery By-product to a Source of Protein and Enzymes
Aspergillus Awamori feed shochu distillery by-product
2009/7/31
Shochu distillery by-product (SDBP) is usually dried before using as an ingredient of formula feed. But drying is costly and causes destruction of the nutrients. In the present study, a new technique ...
Effect of Enzymes of Microbial Origin on in vitro Digestibilities of Dry Matter and Crude Protein in Maize
carbohydrase protease digestibility viscosity maize
2009/7/31
The effects of supplementation of cellulase, xylanase, pectinase, hemicellulase, glucanase, phytase and protease of microbial origins on digestibilities of crude protein (CP) and dry matter (DM) of ma...
Carbohydrases are Digested by Proteases Present in Enzyme Preparations During in vitro Digestion
carbohydrase corn-soybean digestibility Energex protease
2009/7/31
A protease present in an enzyme preparation may digest other enzymes and thus reduces the effectiveness of an enzyme preparation to improve digestibility of animal feeds. Thus the effects of enzyme mi...