搜索结果: 1-15 共查到“知识库 多糖生物化学”相关记录162条 . 查询时间(3.562 秒)
陈兴课题组实现动物活体中的细胞特异性糖质标记与分析（图）
细胞特异性 代谢糖质标记 非天然糖分子 糖质标记和分析
2022/5/7
生物体中几乎所有的细胞都具有相同的基因组，而不同的细胞类型和功能则由不同的基因表达、表观遗传修饰和翻译后修饰等所决定。解析特定器官或组织中特定细胞的生物大分子图谱对于探究发育、细胞间通讯以及疾病的发生发展等都具有重要意义。因此，开发细胞选择性的生物大分子标记方法，近年来受到了科学家们的广泛关注1。通过基因编码的方法，人们在活体动物中实现了蛋白质的组织特异性和细胞选择性标记和分析2,3。然而，糖质（...
蛋白质位点特异性糖基化的精准解析对于研究蛋白质糖基化功能、发现疾病潜在标志物及药物靶标十分重要。大规模、高准确性完整糖肽解析一直是蛋白质后修饰分析领域极具挑战性的工作。近年来，完整糖肽解析在生物质谱分析及计算糖蛋白质组领域都取得了重要进展；然而，目前解析方法仍有明显的局限性：大部分工具无法对糖链鉴定进行质控，尤其是复杂的糖型；检索速度慢；无法对多个糖基化位点精准定位；无法鉴定含有修饰糖单元的完整糖...
蛋白质糖基化修饰的精准分析对于疾病机制研究、生物标志物发现，以及药物和疫苗开发至关重要。完整糖肽分析能获得特定糖基化位点与糖链的对应关系信息，是现代糖蛋白质组学研究中十分重要而极具挑战性的组成部分。目前，糖肽分析最常用的质谱采集模式是数据依赖性采集（DDA），存在母离子选择随机性的问题，其结果中存在较多的缺失值。近年来出现的数据非依赖性采集（DIA）模式能弥补DDA的缺点，已开始初步应用于糖蛋白质...
江南大学生物工程学院产业成果—幽门螺杆菌脂多糖外核心八糖的制备方法。
以柴达木大肥菇(Agaricus bitorquis（Quél.）Sacc)子实体为原料，基于超声波辅助提取手段，采用单因素、响应面优化试验针对超声波提取多糖工艺参数进行优化，结果表明：通过响应面优化提取条件，确定柴达木大肥菇粗多糖提取的最佳工艺为超声功率126 W，料液比1:36 g/mL，提取时间为15 min。采用在此提取工艺条件下提取获得的粗多糖进行动物评价实验，通过小鼠常压耐缺氧、亚硝酸...
Associations between gene polymorphisms, breeding values, and glucose tolerance test parameters in German Holstein sires
Bos taurus milk glucose metabolism DGAT1 GH1 GHR FASN OLR1 ABCG2
2018/11/23
The association between several gene polymorphisms, the estimated breeding values for milk performance traits, and glucose metabolism measured by the glucose tolerance test (GTT) in German Holstein si...
Declining consumption of added sugars and sugar-sweetened beverages in Australia: a challenge for obesity prevention
added sugars Australia food availability obesity sugar-sweetened beverages
2018/11/13
Background: Reduced intakes of added sugars and sugar-sweetened beverages (SSBs) have been the main focus of efforts to stall obesity. Although obesity has risen steeply in Australia, some evidence su...
Mothers’ intake of sugar-containing beverages during pregnancy and body composition of their children during childhood: the Generation R Study
adiposity body composition childhood children cohort epidemiology fetal programming nutrition pregnancy
2018/11/13
Background: High intake of sugar-containing beverages (SCBs) has been linked to increased risk of obesity. However, associations of SCB intake during pregnancy with child body composition have been un...
Effects of Linker Sequences on Vesicle Fusion Mediated by Lipid-Anchored DNA Oligonucleotides
DNA machine synthetic biology
2016/5/25
Synthetic lipid-oligonucleotide conjugates inserted into lipid vesicles mediate fusion when one population of vesicles displays the 5'-coupled conjugate and the other the 3'-coupled conjugate, so that...
Lipid-anchored DNA Mediates Vesicle Fusion as Observed by Lipid and Content Mixing
DNA Vesicles Lipids Fuses
2016/5/25
A general method for synthesizing 5(')- and 3(')-coupled DNA-lipid conjugates has been developed and employed in DNA-mediated vesicle fusion. Vesicles presenting complementary DNA fuse, resulting in b...
Effective Polarity of Frozen Solvent Glasses in the Vicinity of Dipolar Solutes
gene therapy plasmid DNA galactosylated poly(L-lysine) pharmacokinetics hepatic uptake
2016/5/23
The polarity of frozen organic solvent glasses is found to be substantially larger than that of liquid solvents at room temperature. The extent of this increase depends on the dipolar character of bot...
Exercise performed immediately after fructose ingestion enhances fructose oxidation and suppresses fructose storage
de novo lipogenesis energy output fructose gluconeogenesis lactic acid
2018/11/27
Background: Exercise prevents the adverse effects of a high-fructose diet through mechanisms that remain unknown.
桑黄菌丝体多糖的提取及其抗氧化活性研究
桑黄菌丝体 多糖 提取 抗氧化
2016/9/18
研究了桑黄(Phellinus igniarius)菌丝体多糖热水提取的最佳工艺条件及其体外抗氧化活性。通过单因素实验和正交实验对提取工艺进行优化,考察提取时间、温度和料液比及提取次数对桑黄菌丝体多糖得率的影响。最佳提取工艺条件为温度100℃,时间2 h,料液比1∶15 g/m L,提取2次。在该条件下,桑黄菌丝体多糖得率为6.64%。该粗多糖含量为69.29%,蛋白含量为3.20%。浓度为0.2...