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Comparative Cytotoxicity of Fludarabine in Human Peripheral Blood Mononuclear Cells and in the Promyelocytic Leukemia Cell Line (HL60)
HL60 MTT assay fludarabine cytotoxicity differentiation
2009/6/23
Fludarabine is an inhibitor of DNA/RNA synthesis with antitumor activity on lymphoproliferative malignancies. We investigated the dose-dependent cytotoxic effects of the drug on human peripheral blood...
Analysis of Cell-Free Fetal DNA from Maternal Plasma and Serum Using a Conventional Multiplex PCR:Factors Influencing Success
Noninvasive prenatal diagnosis maternal plasma maternal serum fetal gender determination conventional multiplex PCR
2009/6/23
Recent technology enables the use of cell-free fetal DNA in maternal plasma and serum for noninvasive prenatal genetic diagnosis. This study was designed to evaluate factors most likely to influence t...
Cytological and Clinical Features of Hürthle Cell Neoplasia; Review of the Literature Due to Three Cases
Hürthle cell Hürthle cell neoplasm FNA cytology
2009/6/22
Cytological and Clinical Features of Hürthle Cell Neoplasia; Review of the Literature Due to Three Cases.
Are Lymphoblastoid Cell Lines Suitable to Study Increased SMN Gene Expression by Histone Deacetylase Inhibitors?
Spinal muscular atrophy survival motor neuron gene histone deacetylase inhibitors treatment
2009/6/16
Aim: In this preliminary study, we aimed to analyze the effect of sodium butyrate (NB) and phenylbutyrate (PB), belonging to the class of histone deacetylase (HDAC) inhibitors, on lymphoblastoid cell ...
Recognition of T Cell Epitopes Unique to Cha o 2, the Major Allergen in Japanese Cypress Pollen, in Allergic Patients Cross-Reactive to Japanese Cedar and Japanese Cypress Pollen
Cha o 2 cross-reactivity Cry j 2 pollinosis T cell epitope
2010/11/16
Background: Pollens from species of the Cupressaceae family are one of the most important causes of respiratory allergies worldwide. Many patients with pollinosis have specific IgE to both allergens f...
IN VIVO HEMATOPOIETIC CELL ENGRAFTMENT IS MODULATED BY DPPIV/CD26 INHIBITION AND RHEB2 OVEREXPRESSION
Hematopoiesisen Progenitor cells mTOR
2015/5/21
Hematopoietic cell transplantation (HCT) is an important modality used to treat patients with hematologic diseases and malignancies. A better understanding of the biological processes controlling hema...
Reproducibility of Fuhrman Nuclear Grading of Renal Cell Carcinoma: A Preliminary Study
Fuhrman nuclear grade,renal cell carcinoma,reproducibility
2009/5/20
Background: The aim of this study was to evaluate intraobserver and interobserver reproducibility of Fuhrman nuclear grading of renal cell carcinoma. Methods: Pathology slides from 46 cases of renal c...
Nitric oxide acts through different signaling pathways in maturation of cumulus cell-enclosed mouse oocytes
Nitric oxide Sildenafil Oocytes maturation Signaling pathway
2009/12/16
Background: Nitric oxide (NO) have a dual action in mouse oocyte meiotic maturation which depends on its concentration, but the mechanisms by which it influences oocyte maturation has not been exactly...
Analysis of Helper T Cell Responses to Cry j 1-Derived Peptides in Patients with Nasal Allergy: Candidate for Peptide-Based Immunotherapy of Japanese Cedar Pollinosis
Cry j 1 immunotherapy nasal allergy peptide Th response
2010/11/16
Our results suggest four Cry j 1-derived peptides (p61-80, p115-132, p206-225 and p337-353) may be considered to be the immunodominant T-cell epitopes of the Cry j 1 molecule, and can be useful for th...
Annexin A2,A7,and A11 expression in head and neck squamous cell carcinoma
Annexin A2 annexin A7 annexin A11 squamous cell carcinoma head and neck neoplasms
2010/1/12
Aim: To investigate annexin A2 (ANXA2), annexin A7 (ANXA7), and annexin A11 (ANXA11) expression in head and neck squamous cell carcinoma (HNSCC). Although overexpression of ANXA2 has been reported in ...
Human Th17 Cell Clones and Natural Immune Responses
adjuvant cAMP dendritic cells Notch ligand Th17
2010/11/17
Immunomodulators such as lipopolysaccharides (LPS) and forskolin change the nature of dendritic cells (DCs) to induce Th1 and Th2 cells, respectively, thereby designated Th1 or Th2 adjuvants. Recent s...
用间期荧光原位杂交检测卵巢癌细胞中X染色体数目的异常The Application of Interphase Fluorescence in situ Hybridization to the Diagnosis of X Chromosomal Count Abnormality in Ovarian Carcinoma Cell
荧光原位杂交 卵巢癌 X染色体数目异常 DNA特异性探针
2008/1/10
摘要为了探讨用荧光原位杂交技术(fluorescence in situ hybridization, FISH)检测卵巢癌细胞中性染色体拷贝数目异常的实验方法及其应用价值,收集18例新鲜卵巢癌组织标本,以Biotin标记的X染色体α-卫星DNA(pBamX7)探针与经处理的标本进行卵巢癌细胞核的原位杂交,分别用Avidin-FITC和Anti-avidin进行信号的检测与放大,PI复染。于Oly...
CNE1、CNE2鼻咽癌细胞株中ATM/PI3K区基因突变的检测Study on Mutation of ATM/PI3K Region in NPC Cell Lines with Different Radiosensitivity
鼻咽癌 ATM 放射敏感性
2007/12/30
摘要为了探讨具有不同放射敏感性的CNE1、CNE2鼻咽癌细胞株中ATM/PI3K区基因突变的情况,采用反转录聚合酶链式反应(RT-PCR)技术和PCR产物直接测序方法(荧光标记的ddNTPs循环测序),对CNE1、CNE2中ATM的PI3K关键区域进行突变检测。结果表明,所测CNE1、CNE2中的ATM/PI3K区序列中没有发生突变,认为鼻咽癌细胞株CNE1、CNE2间内在的放射敏感性的差异可能与...
The Dynamics of Dendritic Cell—Mediated Innate Immune Regulation
cDCs crosstalk C-type lectin IFN-α pDC
2010/11/18
After taking up pathogen-derived antigens, dendritic cells (DCs) leave peripheral organs and migrate into sentinel lymph nodes via afferent lymphatic vessels. During this process, they undergo maturat...
Dendritic Cells—A Conductor of T Cell Differentiation—
dendritic cells regulatory T cells Th1 Th2 Th17
2010/11/18
Induction of different types of adaptive immune responses depending on the nature of antigens and the environmental context is crucial to cope with a variety of pathogens and concurrently to avoid pat...