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Subpicosecond time-resolved absorption measurements at 77 K on two reaction center (RC) mutants of Rhodobacter capsulatus are reported. In the D(LL) mutant the D helix of the M subunit has been substi...
We present studies on a series of photosynthetic reaction center (RC) mutants created in the background of the Rhodobacter capsulatus D(LL) mutant, in which the D helix of the M subunit has been subst...
Stark spectra have been obtained in the region of absorption by the accessory bacteriochlorophylls (the B-band region around 800 nm) in Rhodobacter sphaeroides reaction centers. The Stark spectra in t...
Resonance Stark effects are nonclassical Stark effects associated with excited-state charge-transfer processes. The theory of resonance Stark effects developed in Part 2 of this series is generalized ...
A theory of the resonance Stark effect data reported in part 1 (preceding paper in this issue) is developed. The model used involves a weak charge resonance interaction between the excited state of an...
Higher order Stark spectroscopy has recently been introduced and applied to characterize the electrooptic properties of chromophores in bacterial photosynthetic reaction centers.1 In the course of the...
The quantum yield of the initial charge separation steps in bacterial photosynthetic reaction centers has been shown to be reduced in an applied electric field [Part I, Lao et al., J. Phys. Chem. 97 (...
The effect of an applied electric field has been measured for the quantum yield of formation of the P[sup +]H[sup [minus]], P[sup +]Q[sub A][sup [minus]], and [sup 3]P states of nonoriented Rb. sphaer...
The rates of electron-transfer reactions can be systematically varied by application of external electric fields. The electric field effect on the charge recombination rate constant of the state P[sup...
Extremely long distance electron transfer is observed in some photosynthetic reaction centers. For several charge recombination reactions, the rate appears to fall off much more slowly with distance t...
A 51 bp section of the Rhodobacter capsulatus photosynthetic reaction center M subunit gene (nucleotides M562-M612 of the pufM structural sequence) encoding amino acids M187-M203 was replaced by the h...
The dependence of the rate constant for electron transfer on energy can be systematically studied by measuring kinetics in an applied electric field. General relationships are developed between observ...
Electron-transfer reactions are expected to be particularly sensitive to externally applied electric fields because of the presence of charge-separated, dipolar states, and the effects of the field on...
The effect of an applied electric field on the kinetics of the initial picosecond electron-transfer reaction in Rb. sphaeroides reaction centers has been measured in isotropic samples at 77 K. The net...
A general method is demonstrated for experimentally obtaining the rate constant of electron transfer as a function of the free energy of the reaction in nonoriented systems. An external electric field...

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