搜索结果: 1-15 共查到“生物学 construction”相关记录25条 . 查询时间(0.065 秒)
CANOPY MODELING OF AQUATIC VEGETATION: CONSTRUCTION OF SUBMERGED VEGETATION INDEX
Remote Sensing Submerged Vegetation Vegetation Index Radiative Transfer Model Sentinel-2A
2018/5/15
The unique spectral characteristics of submerged vegetation in wetlands determine that the conventional terrestrial vegetation index cannot be directly employed to species identification and parameter...
Construction of a Database of Secondary Structure Segments and Short Regions of Disorder and Analysis of Their Properties
Database Short Regions
2015/5/27
Prediction of the secondary structure of a protein from its amino acid sequence
remains an important task. Not only did the growth of database holding only
protein sequences outpace that of solved p...
Embryogenesis in Arabidopsis thaliana: Mutant library construction and embryo mutant identification and characterization
Biological sciences Arabidopsis thaliana Embryogenesis Mutant RSH
2015/1/28
Embryogenesis plays a central role in the plant life cycle. It starts after fertilization. A single zygote divides asymmetrically, giving rise a small apical cell and a larger basal cell. The small ap...
Molecular approaches for the construction of integrated physical and genetic maps
Chromosome microdissection cell lines fiber cells
2014/12/11
This study reports the development of chromosome-specific libraries by chromosome microdissection and microcloning and its utility in developing high density linkage map for particular chromosomes. Ch...
Construction of fat1 Gene Expression Vector and Its Catalysis Efficiency in Bovine Fetal Fibroblast Cells
fat1 Gene Expression Catalysis Efficiency Bovine Fetal Fibroblast Cells
2016/5/9
The FAT-1 protein is an n-3 fatty acid desaturase, which can recognize a range of 18- and 20-carbon n-6 substrates and transform n-6 polyunsaturated fatty acids (PUFAs) into n-3 PUFAs while n-3 PUFAs ...
Beyond the Baldwin Effect: James Mark Baldwin's 'social heredity', epigenetic inheritance and niche construction
niche construction epigenetic inheritance theoretically possible
2011/9/5
I argue that too much attention has been paid to the Baldwin effect. George Gaylord Simpson was probably right when he said that the effect is theoretically possible and may have actually occurred but...
Beyond the Baldwin Effect: James Mark Baldwin's 'social heredity', epigenetic inheritance and niche construction
epigenetic inheritance niche construction evolution developmental genetic
2011/9/8
I argue that too much attention has been paid to the Baldwin effect. George Gaylord Simpson was probably right when he said that the effect is theoretically possible and may have actually occurred but...
Construction of Mammalian Cell Expression Vector for pAcGFP-bFLIP(L) Fusion Protein and Its Expression in Follicular Granulosa Cells
c-FLIP(L) pAcGFP-N l Recombinant Plasmid Follicular Granulosa Cell
2016/5/4
FLICE inhibitory protein (FLIP) is one of the important anti-apoptotic proteins in the Fas/FasL apoptotic path which has death effect domains, mimicking the pro-domain of procaspase-8. To reveal the i...
甲型肝炎病毒衣壳蛋白融合基因植物表达载体的构建与遗传转化柑桔的研究Construction of the Plant Expression Vector with Hepatitis a Capsid Protein Fusion Gene and Genetic Transformation of Citrus.Sinensis Osbeck
甲型肝炎病毒衣壳蛋白融合基因 柑桔 植物表达载体 遗传转化 食用疫苗
2008/1/20
摘要
基因工程领域的研究进展使得植物体成为具有重要经济价值的药用蛋白的生产体系。以含甲型肝炎病毒结构基因cDNA的克隆载体pCDNAⅡA16为模板，用甲型肝炎病毒衣壳蛋白融合基因特异引物进行PCR扩增，得到全长2.2kb衣壳蛋白融合基因序列。经测序鉴定后正向克隆于植物表达载体pBI121中，衣壳蛋白融合基因位于pBI121质粒T-DNA左右边界区间内，处于CaMV35S启动子控制之下。经限制性内...
鸡解偶联蛋白(UCP)基因内含子的克隆与系统发生树的构建Cloning and Sequencing of the Introns of UCP Gene and Construction of Molecular Phylogenetic Tree in Chicken
鸡 解偶联蛋白基因 分子系统发生树
2008/1/10
摘要 解偶联蛋白基因是新近发现的能够增加能量的消耗，与脂肪代谢和能量调控密切相关的一组基因。本研究根据小鼠UCP2基因的剪切方式，设计4对引物成功克隆测序了鸡UCP基因的全部5个内含子，发现都是GT-AG类型的内含子，鸡UCP基因的结构和小鼠的UCP2基因结构一致。以不同物种UCP基因的cds 区域序列和内含子2、内含子3序列进行系统发生树的构建，结果表明：以UCP基因cds区域序列构建的系统发生...
一步3’RACE快速构建鸡MnSOD全长cDNA克隆Rapid Construction of Full-length MnSOD cDNA of Chickens by One-step 3’RACE
RACE 触减PCR MnSOD cDNA
2008/1/8
摘要
本研究尝试将触减 PCR与3’ cDNA末端快速扩增（rapid amplification of cDNA ends，RACE）技术进行结合，仅用一条特异性引物和一条通用引物，成功地实现了从3’末端cDNA库对鸡含锰超氧化物歧化酶（manganese-containing superoxide dismutase，MnSOD）全长cDNA的一步3’RACE快速构建。与常规使用的末端PCR...
鉴定痢疾杆菌毒力基因的SW480细胞模型构建Construction of SW480 Cell Model Identifying Shigella Virulent Genes
SW480 细胞模型 痢疾杆菌 信号标签诱变技术 构建
2008/1/8
摘要
信号标签诱变技术（STM）是一种在体内高通量筛选病原体毒力基因的新方法，在应用时的一个先决条件是要建立合适的体内筛选系统。为将该技术应用于福氏痢疾杆菌，我们使用三个福氏痢疾杆菌菌株进行了预试验：通过同源重组构建而成的带有氯霉素抗性且aroA和virG基因失活的突变株RC426；因在侵袭质粒上自发缺失3个基因座(ipaBCDA, invA 和 virG)的另一减毒突变株T32，其曾被用作福氏...
灰树花总DNA的制备及基因组文库的构建A Method for Preparation of Genomic DNA FromGrifola frondosa and Construction of a Genomic Library
灰树花 DNA提取 基因组文库
2008/1/8
摘要
灰树花是一种珍贵的药用真菌，因为多糖含量较高，较难获得高质量的总DNA，本文提出了一种制备高质量灰树花总DNA及构建灰树花基因组文库的方法。该方法制备的灰树花总DNA，经Sau3AI酶切后，用于构建基因组文库，可得到2×105个转化子/50mg，平均插入片段为14kb。本研究为下一步克隆灰树花中的基因以及进行其他分子生物学研究奠定了基础。Abstract： Grifola frondos...
苦瓜MAP30基因原核表达载体的构建和PCR快速检测重组子的研究 Construction of Prokaryotic Expression Vector for MAP30 Gene and Study of PCR Methods for Rapid Identification of Recombinant
MAP30 PCR 克隆子筛选 苦瓜
2008/1/8
摘要
根据已报道的序列，把苦瓜(Momordica charantia) MAP30基因成功克隆到原核表达载体pET28a（+）中，并对PCR快速检测阳性克隆进行了研究。结果表明，直接用菌落、菌液、酚氯仿处理过的菌液,以及提取的质粒进行PCR都可以成功地筛选阳性菌落。其中，酚氯仿处理过的菌液PCR与质粒PCR的结果最接近，而且比质粒PCR简单，因此可作为方便可靠的阳性克隆筛选的新方法。Abstr...
番茄转录调控因子基因Pti5植物表达载体的构建及其转化烟草的研究Construction of a Plant Expression Vector with Tomato Transcription Factor Gene Pti5 and Studies on Transgenic Tobaccos
Pti5-VP16基因 表达载体 转化 烟草
2007/12/31
摘要本实验构建了含有CaMV35S启动子控制下的Pti5-VP16基因的植物双元表达载体pBI121UCH1。通过根癌农杆菌叶盘转化法，将Pti5-VP16基因导入烟草SRI中，经卡那霉素筛选，获得了抗性植株。经PCR和Southern印迹分析，表明抗性植株中整合了Pti5-VP16基因，经抗病性鉴定转基因烟草植株的抗病性明显提高。
Abstract:The plasmid pBI121UCH1...