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Effects of protein supplementation combined with resistance exercise on body composition and physical function in older adults: a systematic review and meta-analysis
elderly people obesity protein supplementation resistance exercise body composition
2018/11/16
Background: Overweight and obese older people face a high risk of muscle loss and impaired physical function, which may contribute to sarcopenic obesity. Resistance exercise training (RET) has a benef...
Effects of protein supplementation combined with resistance exercise on body composition and physical function in older adults: a systematic review and meta-analysis
elderly people obesity protein supplementation resistance exercise body composition
2018/11/20
Background: Overweight and obese older people face a high risk of muscle loss and impaired physical function, which may contribute to sarcopenic obesity. Resistance exercise training (RET) has a benef...
The role of gluten consumption at an early age in celiac disease development: a further analysis of the prospective PreventCD cohort study
celiac disease children gluten HLA European cohort
2018/11/13
Background: We previously found that the introduction of small quantities of gluten at 4–6 mo of age did not reduce the risk of celiac disease (CD) in a group of high-risk children. However, the consu...
Formation and Analysis of Topographical Domains Between Lipid Membranes Tethered by DNA Hybrids of Different Lengths
Cell Membrane DNA Membrane Lipids Lipid Bilayers 1,2-Dipalmitoylphosphatidylcholine Microscopy, Fluorescence Base Sequence Molecular Sequence Data Models,Chemical Unilamellar Liposomes
2016/5/25
We recently described a strategy to prepare DNA-tethered lipid membranes either to fixed DNA on a surface or to DNA displayed on a supported bilayer [Boxer et al., J. Struct. Biol., 2009, 168, 190; Bo...
Structural and Spectroscopic Analysis of the Kinase Inhibitor Bosutinib and an Isomer of Bosutinib Binding to the Abl Tyrosine Kinase Domain
Humans Escherichia coli Benzamides Quinolines Nitriles Aniline Compounds Piperazines Pyrimidines Thiazoles Recombinant Proteins
2016/5/25
Chronic myeloid leukemia (CML) is caused by the kinase activity of the BCR-Abl fusion protein. The Abl inhibitors imatinib, nilotinib and dasatinib are currently used to treat CML, but resistance to t...
Anomalous Negative Fluorescence Anisotropy in Yellow Fluorescent Protein (YFP 10C):Quantitative Analysis of FRET in YFP Dimers
Alzheimer's disease Amyloid beta 40 peptide Human serum albumin Circular dichroism Dissociation constant alpha-Helix
2016/5/25
Yellow fluorescent protein (YFP) is widely used as a genetically encoded fluorescent marker in biology. In the course of a comprehensive study of this protein, we observed an unusual, negative fluores...
Quantitative Analysis of Supported Membrane Composition Using the NanoSIMS
lipid bilayer SIMS NanoSIMS mixture gradient compositional analysis
2016/5/24
We have improved methods reported earlier [1] for sample preparation, imaging and quantifying components in supported lipid bilayers using high-resolution secondary ion mass spectrometry performed wit...
Analysis of the Spatiotemporal Localization of Mitochondrial DNA Polymerases of Trypanosoma brucei
cell cycle DNA polymerase kinetoplast DNA microscopy mitochondrion Trypanosomes
2015/1/28
The mitochondrion contains its own genome. Replication of the mitochondrial DNA (mtDNA) is an essential process that, in most organisms, occurs through the cell cycle with no known mechanism to ensure...
Analysis of the interaction between the epidermal growth factor receptor and actin
Epidermal growth factor protein amino acids growth factors actin cell free system cytosol cells actin immune
2014/12/11
It has been shown that the epidermal growth factor receptor (EGFR) can directly interact with F-actin via an actin-binding domain (ABD) located at amino acid residues 984–996. In this study, we invest...
The stability of T7 RNA polymerase elongation complexes and promoter function analysis by in vitro selection
Forward translocation model polymerase complex stability DNA structure
2014/12/11
The forward translocation model (paused or stalled polymerase moves forward in the absence of transcription, dissociation of the complex happens when hybrid is too short to stabilize the complex) is t...
Functional analysis of Hic-5/ARA55 isoforms in C2C12 myogenesis
protein protein cell clones hydrogen peroxide androgen receptor activator
2014/12/11
Hic-5 is a focal adhesion protein of paxillin superfamily that was initially cloned from mouse osteoblasts as a TGF-β or H2O2 inducible cDNA. As well, Hic-5 was independently identified as an Androgen...
Functional analysis of Moe (Epb4.1l5) in zebrafish development and the identification of novel Epb4.1l5 binding proteins
Zebrafish Protein Mammals Red blood cell protein Embryonic development. The texture of retina
2014/12/11
The zebrafish protein Mosaic eyes (Moe), and the mammalian orthologue Erythrocyte protein band 4.1-like 5 (Epb4.1l5), are FERM (for Protein 4.1, Ezrin, Radixin, Moesin) domain containing proteins that...
MU researchers offer first analysis of new human glucose disorder
human glucose disorder MU researchers
2014/12/1
COLUMBIA, Mo. – Glycogen storage disorders, which affect the body’s ability to process sugar and store energy, are rare metabolic conditions that frequently manifest in the first years of life. Often ...
ANALYSIS OF THE INTERACTION BETWEEN TOMATO TORRADO VIRUS PROTEINS USING THE YEAST TWO-HYBRID SYSTEM
gateway strategy Tomato torrado virus viral proteins interaction yeast two-hybrid system
2014/2/21
Ten years ago for the first time the new picorna-like virus species – Tomato torrado virus (ToTV) – was found and described
on tomato plants. The isolates of this pathogen were reported in Europe, A...
Single-enzyme analysis in a droplet-based micro-and nanofluidic system
Single-enzyme analysis droplet micro nanofluidic system
2016/12/2
The kinetic activity of individual enzyme molecules was determined in aqueous droplets generated in a nano- and microfluidic device. To avoid high background noise, the enzyme and substrate solution w...