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Assessing the Importance of Sand as a Source of Fecal Indicator Bacteria (Escherichia coli and Enterococcus)
Escherichia coli Enterococcus
2015/7/21
The purpose of this activity is for students to investigate water quality, and the factors that affect it, at a local beach. This is accomplished by (1) measuring concentrations of Escherichia coli an...
Replication restart in Escherichia coli: Genetic studies at the interface of recombination and replication
DNA replication forks DNA damage proteins cells enzyme catalysis
2015/1/28
DNA replication forks are frequently detained during their progression from oriC to the replication terminus due to encounters with DNA lesions or proteins blocking the way. For survival, cells deploy...
Oxidative stress responses in Escherichia coli and Pseudomonas aeruginosa
Aerobic respiration reactive oxygen species ROS damage biological molecules cells detoxification oxidative stress transcription factors signal differential gene expression escherichia coli pseudomonas aeruginosa
2015/1/28
Aerobic respiration generates unavoidable reactive oxygen species (ROS, detrimental by-products that damage biological molecules. The accumulation of ROS beyond the cellular capacity for detoxificatio...
The SOS response in Escherichia coli: Single cell analysis using fluorescence microscopy
Biological sciences DNA repair RecA SOS response
2015/1/28
During the course of DNA replication, replication forks often stall or collapse as they proceed from oriC to the terminus due to housekeeping types of DNA damage or proteins bound to DNA. If the DNA i...
Reca Dynamics & the SOS Response in Escherichia Coli: Cellular Limitation of Inducing Filaments
DNA damage RadA RecA SOS response
2015/1/28
During the course of normal DNA replication, replication forks are constantly encountering "housekeeping" types of routine damage to the DNA template that may cause the forks to stall or collapse. One...
Differential afferent sensitivity to mucosal lipopolysaccharide from Salmonella typhimurium and Escherichia coli in the rat jejunum
5-HT afferent nerve enteritis hypersensitivity mesenteric
2014/5/16
Postinfectious irritable bowel syndrome may develop subsequent to acute bacterial enteritis. We therefore hypothesized that intestinal afferents may develop hypersensitivity upon exposure to luminal l...
改进大肠杆菌（E. coli）局限性转导试验的探索1)
大肠杆菌 局限性 转导试验
2008/1/18
在大肠杆菌吓·coli）局限性转导（restricted transduction')实验中，通常用A噬菌体特异性转导半乳糖发酵基因（gal的现象来说明转导的基本原理和掌握转导实验的基本方法。近年来，我们在指导学生进行这一实验过程中，对实验菌株的选择、噬菌体（.L)的诱导、噬菌体裂解液的教价测定和进一步提高转导频率等方面进行了一些改进，收到了较好的效果。本文扼要介绍我们的实验结果。
大肠杆菌FC40系统静止期突变中的F因子转移The Occurrence of Actual F Factor Transfer during the Stationary-phase Mutation in Escherichia coli FC40
大肠杆菌FC40系统 适应性突变 F因子转移
2007/12/31
摘要本研究采用大肠杆菌GM133 rifr细胞和营养收集细胞HB214 strr进行适应性突变实验。在混合30min和2d 后添加链霉素杀死GM133基因型细胞，继续培养5d后，在选择平板上出现了一定数量的lac+strr基因型回复突变菌落。根据这些突变菌落的数量，估计在lac+突变产生之前，GM133和HB214细胞之间的接合频率分别为0.07%和7.47%。在培养了7d的选择平板上添加含链霉素...
摘要 纳豆激酶是一种纤维蛋白溶解酶 ,有望开发成为新型的溶栓药物 .从中国豆豉中分离的具有较强纤溶活性的枯草杆菌DC 2中提取总DNA ,根据纳豆激酶 (NK)基因序列设计引物 ,用PCR法扩增NK基因 .序列分析表明 ,NK基因成熟肽编码区含有 82 5bp ,编码 2 75个氨基酸残基 ,与文献报道的序列分别有 93 4 %和 94 5 %同源性 .将NK基因插入载体pGEX 4T1构建表达...
传染性支气管炎病毒核衣壳蛋白基因克隆及在E.coli中的表达
传染性支气管炎病毒 核衣壳蛋白基因 克隆 非融合表达
2007/12/20
摘要 核衣壳蛋白基因 (N基因 )是传染性支气管炎病毒的重要结构基因 .根据已报道的序列设计引物 ,利用RT PCR技术从病毒RNA中扩增和克隆到了N基因的cDNA ,并测定了核苷酸序列 .克隆的N基因片段ORF全长 12 30bp ,编码 4 0 9个氨基酸 .将该片段序列与其他IBV病毒株比较 ,核苷酸的同一性为 87 0 %～ 98 6 %,氨基酸的同一性为 91 0 %～ 98 1...
苏云金杆菌以色列亚种cryIVB基因在E.coli中的高表达
Ｂｔｉ ｃｒｙＩＶＢ 基因表达 韭菜迟眼蕈蚊
2007/12/18
摘要 韭菜迟眼蕈蚊（Ｂｒａｄｙｓｉａｏｄｏｒｉｐｈａｇａ）幼虫以取食根部危害韭菜的生长．为了研究有效的生防制剂，采用ＤＮＡ聚合酶链式反应方法，从苏云金杆菌以色列亚种（Ｂｔｉ）得到３．５ｋｂ的开放阅读框架（ＯＲＦ），其包括Ｂｔｉ杀虫蛋白基因ｃｒｙＩＶＢ的结构基因和部分上游启动区．将结构基因亚克隆到高表达载体ｐＱＥ５２中，构建了高表达质粒ｐＱＥ５２Ｂ．在Ｅ．ｃｏｌｉＳＧ１３００９（ｐＲＥＰ４）中，经Ｉ...
大肠埃希菌氨基糖苷类耐药株aac(3)Ⅱ基因保守区分析Conserved Region Analysis of aac(3)ⅡGene From E.coli Aminoglycoside Resistance Strain
温州医学院检验与公共卫生学院 细胞与分子医学研究所 温州
2007/12/7
摘要常规方法分离鉴定47株大肠埃希菌，以标准纸片扩散法(KB法)对其进行常用氨基糖苷类药物敏感性测定，耐药株经PCR检测aac(3)Ⅱ基因保守区，扩增产物进行DNA测序分析。初步探讨大肠埃希菌氨基糖苷类抗生素耐药株与aac(3)Ⅱ基因保守区之间的关系，结果显示本地区大肠埃希菌氨基糖苷类抗生素耐药株的aac(3)Ⅱ基因保守区具65位G、84位T和65位A、84位C两种基因型，且高...