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LAMP技术快速检测海产品副溶血弧菌的条件优化
副溶血弧菌 LAMP 海产品 tlh
2016/5/27
本研究以副溶血弧菌不耐热溶血素tlh为目标基因设计特异性引物,优化反应条件,并对特异性、灵敏度进行了验证。LAMP最佳反应条件为,外内引物浓度比为1∶8,Mg2+反应浓度为4 mmol/L,d NTPs浓度为3.5 mmol/L,温度为65℃,反应时长为1 h,只对副溶血弧菌产生扩增反应,与其余细菌不产生扩增反应,细菌基因组DNA和纯培养物的灵敏度约为5.45×101 fg/μL和140 cfu/...
Geometric Modelling of Octagonal Lamp Poles
LiDAR Octagonal Pole Geometric Modelling Polygon
2014/7/30
Lamp poles are one of the most abundant highway and community components in modern cities. Their supporting parts are primarily tapered octagonal cones specifically designed for wind resistance. The g...
应用LAMP方法检测动物源性食品中沙门菌
环介导等温扩增技术 沙门菌 动物源性食品
2015/12/30
运用两种方法对动物源性食品中沙门菌进行检测，找出一种更加快速、敏感、特异、简便的检验方法。方法 采集了60份市售的禽、畜等生肉及60份奶制品，分别采用LAMP方法和国家标准方法GB/T 4789.4-2008对沙门菌进行分离与鉴定。结果 120份采集样中，LAMP方法检出2例，阳性率1.67 %；国家标准方法检出1例，阳性率0.83 %。LAMP方法的符合率为99.2 %，敏感性为100 %，特异...
基于LED诱虫灯的果蝇趋光性试验（Experiment of Light Emitting Diode Catching Insect Lamp on Phototaxis of Drosophila）
果蝇 LED诱虫灯 趋光性
2009/11/3
采用固体冷光源发光二极管（LED），设计了可以调控LED光源光密度、闪烁频率和波长的柔性诱虫灯，并对果蝇进行了趋光性试验。结果表明，果蝇趋性最强的光源波长为560 nm，光强弱的黄绿光诱捕效果最好。
Atmospheric Pressure Deposition of Silica Thin Film by Photo-CVD Using Vacuum Ultraviolet Excimer Lamp
Excimer lamp Silica thin film TEOS
2009/6/8
It has been known that thin silica films can be deposited from TEOS {Si(OC2H5)4} by means of the photo-CVD (Chemical Vapor Deposition) technique using vacuum ultraviolet (VUV) light (VUV-CVD) at room ...
Using a halogen lamp to calibrate an optical system for UV-VIS radiation detection
calibration optical system patron sources
2011/5/5
In the last few years plasmas have been used more and more in a great number of scientific and technological fields, and optical emission spectroscopy (OES) has been the most commonly used technique f...