搜索结果: 1-2 共查到“家畜繁殖学 RT-PCR”相关记录2条 . 查询时间(0.109 秒)
一例猪繁殖与呼吸综合征的RT-PCR诊断与防制
猪繁殖与呼吸综合征病毒 细胞培养 Marc-145 RT-PCR 诊断 防制
2009/11/5
2008年3月，新乡市某猪场发生了以一种经产母猪和初产母猪流产、死产、产木乃伊胎、死胎及弱仔为特征的疾病。采集流产胎儿和死胎的肺、肝、脾脏等病料，制悬液，取上清，进行细胞培养，观察细胞病变。另根据猪繁殖与呼吸综合征病毒美洲株VR-2332 ORF7基因序列设计一对特异引物，提取流产胎儿的肺、肝等组织中PRRSV的RNA，经RT-PCR扩增，检测猪繁殖与呼吸综合征病毒为阳性，结合病史和临床症状，诊断...
Identification of Spermatogenic Cells Expressing Protamine mRNA in Japanese Quail by RT-PCR
micropipetting protamine mRNA quail RT-PCR spermatogenic cells
2009/7/31
This study was conducted by reverse transcription-polymerase chain reaction (RT-PCR) analysis to determine when the protamine mRNA is expressed in testicular germ cells during spermatogenesis in quail...