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Expression of cyclin-dependent kinase inhibitors (CDKN1, CDKN5) in developmentally competent and incompetent porcine oocytes
Cdkn1 Cdkn5 oocyte developmental competence
2015/4/16
Although several reports have been published regarding the cyclin-dependent kinases (Cdks) in developmentally competent mammalian oocytes, still little is known about their expression pattern in devel...
FSH和17β-雌二醇联合作用对Cyclin D1 mRNA和Cyclin E1 mRNA表达的影响
FSH 17β-雌二醇 睾丸支持细胞 细胞周期蛋白D1 细胞周期蛋白E1 细胞外信号调节的蛋白激酶1/2
2013/11/28
为了确定FSH和雌激素联合作用对培养条件下未成熟仔猪睾丸支持细胞中Cyclin D1 mRNA和Cyclin E1 mRNA表达的影响。以培养的仔猪睾丸支持细胞为研究材料，通过添加各种信号通路的抑制剂，应用实时荧光定量PCR检测Cyclin D1 mRNA和Cyclin E1 mRNA的相对表达量。FSH(50 ng·mL-1)和17β-雌二醇 (10-9 mol·L-1)联合作用时对Cyclin...