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Cryopreservation of fluorescence activated cell sorted boar spermatozoa based on extracellular ubiquitination
pig antibody staining quality marker porcine sperm FACS ubiquitin cryopreservation
2016/8/1
The present study is focused on the methodology of fluorescence activated cell sorting (FACS) of spermatozoa stained by the antibody against extracellular surface marker ubiquitin (eUb) and subsequent...
Kinetics of hydrogen peroxide generated from live and dead ram spermatozoa and the effects of catalase and oxidase substrates addition
reactive oxygen species ram antioxidant
2016/1/29
The generation of hydrogen peroxide (H2O2) by ram spermatozoa (spz) was measured using a flurometric assay with 10-acetyl-3,7-dihydroxyphenoxazine agent as a probe for H2O2 detection. The kinetics of ...
Effect of long-term storage on induced photon emission of boar spermatozoa
boar semen oxidative stress luminescence photon emission sperm motility
2015/6/2
Ultraweak photon emission measurements were found to be a useful tool for investigating the influence of stress factors on cells. In the present study, induced photon emission of boar spermatozoa was ...
DNA integrity in fresh, chilled and frozen-thawed canine spermatozoa
dog DNA fragmentation index sperm chromatin structure assay (SCSA) cryopreservation chilled semen
2015/4/16
Sperm chromatin status in fresh dog semen and the effect of long-term storage of chilled and frozen dog semen on sperm chromatin integrity was assessed by the sperm chromatin structure assay (SCSA). I...
Determination of Acrosomal Proteolytic Activity in Spermatozoa Collected from Quail Treated with Diethylstilbestrol,Using Gelatin-Substrate Slide Technique
acrosomal proteolytic activity (APA) diethylstilbestrol gelatin-substrate slide quail sperm
2009/7/30
The present experiment was designed to ascertain whether gelatin-substrate slide technique devised for detection of the proteolytic activity of acrosomal contents can be used for quail sperm, and to d...