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Comparative Cytotoxicity of Fludarabine in Human Peripheral Blood Mononuclear Cells and in the Promyelocytic Leukemia Cell Line (HL60)
HL60 MTT assay fludarabine cytotoxicity differentiation
2009/6/23
Fludarabine is an inhibitor of DNA/RNA synthesis with antitumor activity on lymphoproliferative malignancies. We investigated the dose-dependent cytotoxic effects of the drug on human peripheral blood...
Adhesion of Beta1 Integrin to Fibronectin Regulates CAM-DR Phenotype via p21WAF1/cip1 in HL60 Acute Myeloid Leukemia (AML) Cells
Apoptosis CAM-DR fibronectin cell cycle
2009/6/16
Aims: Drug resistance is a major obstacle for a successful cancer therapy. Cell adhesion mediated drug resistance (CAM-DR) is a novel type of drug resistance and generated via interaction of cancer ce...
人白血病HL60细胞的分化状态对细胞凋亡的影响
细胞分化 细胞凋亡 三尖杉酯碱 人白血病HL60细胞 c-myc基因
2010/2/25
用细胞培养和流式细胞术等方法,研究人白血病HL60细胞诱导分化后,对三尖杉酯碱(Har)和喜树碱(Cam)诱导细胞凋亡的影响。结果表明,12-豆蔻酰及13-乙酸佛波酯以16nmol·L-1浓度处理HL60细胞24h,细胞向单核/巨噬细胞方向分化,阻断于G1期;分化细胞抗Har和Cam诱导的细胞凋亡,但其c-myc基因的表达无变化。1.4%二甲基亚砜处理HL60细胞48h,细胞向粒细胞方向分化,阻断...