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Detection of Mutation in 23S rRNA in Isolates of Streptococcus pyogenes Resistant to Erythromycin
Streptococcus pyogenes 23S rRNA erythromycin-resistance
2009/12/17
Background: In this study, erythromycin resistance isolates from the students with aged 12-15 yr old were studied and the mutations in the 23S rRNA were identified by sequencing.Methods: Throat sample...
The Use of a Nonradioactive Digoxigenin Labeled Probe for Detection of Apo(a) Gene Size
Lp(a) coronary artery disease dot blot analysis DIG-labeling apo(a) gene
2009/6/30
Elevated lipoprotein(a) [Lp(a)] concentrations are associated with premature coronary heart disease and early myocardial infarction. In the general human population the sizes of apolipoprotein [apo(a)...
Detection of the SRY Gene in a 46,XY Phenotypic Female by the PCR-SSCP Method
PCR-SSCP Sex reversal SRY 46 XY Female
2009/6/30
The short arm of the human Y chromosome determines maleness, initiating the development of the testes. Mutations and deletions in this region affect gonadal differentiation, causing primary sex revers...
Detection of Human Cytomegalovirus Gene in Formalin-Fixed Paraffin-Embedded Fetal Tissues
Diagnostic application of the ligation-dependant polymerase chain reaction
2009/6/26
Cytomegalovirus (CMV) is an opportunistic pathogen that can cause infection at any time during the course of a lifetime and constitutes an important cause of intrauterine infection and death in newbor...
Evaluation of the Efficacy of Five DNA Extraction Methods for the Detection of Mycobacterium tuberculosis DNA in Direct and Processed Sputum by an In-House PCR Method
DNA extraction Mycobacterium tuberculosis PCR
2009/5/20
Aim: DNA extraction is an important step from clinical samples for molecular diagnosis of tuberculosis by PCR. The aim of this study was to evaluate the efficacy of five DNA extraction methods [boil...
PCR及其衍生技术在基因突变检测中的应用Use of PCR Related Methods in Detection of Gene Mutation
基因敏感性 RNA 染色质重组 PCR 衰老
2007/12/30
摘要许多人类遗传性疾病及某些抗艾滋病药物的抗性乃至细菌对某些抗生素的抗药性通常源于基因突变。本文对近年来在基因突变检测中应用日益广泛的各种PCR 衍生技术作一综述;重点介绍了错配PCR技术,以及我们实验室近期报道的一种快速检测喹诺酮类药物耐药大肠杆菌的错配PCR方案。
Abstract:Many inherited diseases and drug resistance have been a...