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Protein secretion by the rat epididymis can be selectively modified in vitro by local anesthetics, glucose deprivation, dinitrophenol, ouabain, and ionophores
Protein secretion rat epididymis selectively modified local anesthetics
2009/2/20
Protein secretion by the caput epididymidis has been examined in vitro using radioactive methionine as a precursor for protein synthesis. Newly synthesized and secreted proteins were separated by gel ...
The male antifertility agents alpha chlorohydrin, 5-thio-D-glucose, and 6-chloro-6-deoxy-D-glucose interfere with sugar transport across the epithelium of the rat caput epididymidis
rat epididymis sugar transport antifertility
2009/2/19
The effects of the male antifertility agents alpha-chlorohydrin, 5-thio-D-glucose, and 6-chloro-6-deoxy-D-glucose on sugar transport (3H-3-O-methyl-D-glucose and 3H-2-deoxy-D-glucose) across the r...
A Continuous Flow Method for Organ Culture of Rabbit Epididymis:Morphology, Amino Acid Utilization, Glucose Uptake, RNA, and Protein Synthesis
epididymis organ culture glucose uptake amino acid utilization
2009/2/18
A modified perfusion system was developed to maintain an unconvoluted tubule from the corpus epididymidis of the rabbit in organ culture. The epididymal explant is submerged in a continuous flow of ...
An improved mouse sperm-oocyte plasmalemma binding assay: studies on characteristics of sperm binding in medium with or without glucose
Zona-free oocytes pronuclear zygotes in vitro fertilization
2009/2/18
Sperm binding to the oocyte plasmalemma is crucial to subsequent steps in fertilization. However, the usual in vitro assay for sperm-oocyte binding does not distinguish between nonspecific attachmen...
Effects of glucose and other energy substrates on the hyperactivated motility of macaque sperm and the zona pellucida-induced acrosome reaction
glucose hyperactivated motility macaque sperm
2009/2/13
Energy sources in sperm capacitation media have various effects on mammalian sperm and are required for stimulation of hyperactivated motility and/or acrosome reactions in some species. The presen...
The role of glucose in supporting motility and capacitation in human spermatozoa
Acrosome reaction ATP hamster oocyte penetration
2008/12/29
Glucose has been reported to be beneficial to human sperm for optimal capacitation and fertilization, although it is unclear whether glucose is required for providing extra metabolic energy through gl...