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Covalent Attachment of Lipid Vesicles to a Fluid-Supported Bilayer Allows Observation of DNA-Mediated Vesicle Interactions
Caspian and Black Sea waters reverse-osmotic desalination Na-zeolite softening of the permeate
2016/5/25
Specific membrane interactions such as lipid vesicle docking and fusion can be mediated by synthetic DNA-lipid conjugates as a model for the protein-driven processes that are ubiquitous in biological ...
DNA-tethered Membranes Formed by Giant Vesicle Rupture
Tethered lipid membrane Surface immobilized DNA Giant unilamellar vesicles
2016/5/25
We have developed a strategy for preparing tethered lipid bilayer membrane patches on solid surfaces by DNA hybridization. In this way, the tethered membrane patch is held at a controllable distance f...
Effects of Linker Sequences on Vesicle Fusion Mediated by Lipid-Anchored DNA Oligonucleotides
DNA machine synthetic biology
2016/5/25
Synthetic lipid-oligonucleotide conjugates inserted into lipid vesicles mediate fusion when one population of vesicles displays the 5'-coupled conjugate and the other the 3'-coupled conjugate, so that...
Lipid-anchored DNA Mediates Vesicle Fusion as Observed by Lipid and Content Mixing
DNA Vesicles Lipids Fuses
2016/5/25
A general method for synthesizing 5(')- and 3(')-coupled DNA-lipid conjugates has been developed and employed in DNA-mediated vesicle fusion. Vesicles presenting complementary DNA fuse, resulting in b...
Kinetics of DNA-mediated Docking Reactions Between Vesicles Tethered To Supported Lipid Bilayers
DNA electron transfer oxidative stress redox regulation
2016/5/25
Membrane-membrane recognition and binding are crucial in many biological processes. We report an approach to studying the dynamics of such reactions by using DNA-tethered vesicles as a general scaffol...
Spatially localized generation of nucleotide sequence-specific DNA damage
sperm chemotaxis fertilization TPX-1 AEG CRISP
2016/5/23
Psoralens linked to triplex-forming oligonucleotides (psoTFOs) have been used in conjunction with laser-induced two-photon excitation (TPE) to damage a specific DNA target sequence. To demonstrate tha...
Electrophoresis of DNA Adsorbed to a Cationic Supported Bilayer
hydrogen absorbing materials rare earth alloys gas-solid reactions order-disorder effects neutron diffraction
2016/5/23
We report fluorescence microscopy studies of the electrophoresis of individual DNA molecules electrostatically adsorbed to a cationic supported lipid bilayer. Obstacles to uniform electrophoretic flow...
Multi-nuclear NMR Studies of DNA Hairpins:2.Sequence-Dependent Structural Variations
GERMANIUM DETERMINATION HYDRIDE GENERATION INDUCTIVELY COUPLED PLASMA EMISSION SPECTROMETRY MEDICINAL PLANT
2016/5/20
The solution conformation of three related DNA hairpins, each with five bases in the loop, is investigated by proton and phosphorus 2D NMR methods. The sequences of the three oligomers are d(CGCGTTGTT...
Multi-nuclear NMR Studies of DNA Hairpins:1. Structure and Dynamics of d(CGCGTTGTTCGCG)
GERMANIUM DETERMINATION HYDRIDE GENERATION INDUCTIVELY COUPLED PLASMA EMISSION SPECTROMETRY MEDICINAL PLANT
2016/5/20
The solution structure of the hairpin formed by d(CGCGTTGTTCGCG) has been examined in detail by a wide variety of NMR techniques. The hairpin was characterized by proton NMR to obtain interproton dist...
Synthesis of a Thymidine Phosphoramidite Labelled with 13C at C6: Relaxation Studies of the Loop Region in a 13C Labelled DNA Hairpin
Thymidine Phosphoramidite Labelled 13C C6 Relaxation Studies 13C Labelled DNA Hairpin
2016/5/20
A thymidine phosphoramidite labelled at C6 with 13C has been synthesized, and incorporated into a synthetic oligonucleotide, d(CGCGT*T*GT*T*CGCG), which adopts a hairpin conformation. NMR relaxation m...
Breed Discrimination Using DNA Markers Derived from AFLP in Japanese Beef Cattle
AFLP Breed Discrimination Beef Cattle Japanese Black Holstein
2016/4/21
In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. This paper describes the developm...
Accuracy of Estimating Genetic Distance between Species from Short Sequences of Mitochondrial DNA
Estimating Genetic Distance Mitochondrial DNA
2015/12/17
Recent advances in molecular techniques allow rapid determination of genetic
divergence, and in many circumstances the polymerase chain reaction (PCR) and
DNA sequencing have become the technologi...
Rapid Production of Single-Stranded Sequencing Template from Amplified DNA Using Magnetic Beads
Single-Stranded Sequencing Magnetic Beads
2015/12/17
We present a powerful, general method for producing a pure preparation of single-stranded DNA (ssDNA) sequencing template from doublestranded DNA (dsDNA) amplified using the polymerase chain reaction ...
Pre-Whaling Genetic Diversity and Population Ecology in Eastern Pacific Gray Whales: Insights from Ancient DNA and Stable Isotopes
Population Ecology Pre-Whaling Genetic Diversity
2015/12/11
Commercial whaling decimated many whale populations, including the eastern Pacific gray whale, but little is known about
how population dynamics or ecology differed prior to these removals. Of partic...
Class prediction by nearest shrunken centroids,with applications to DNA microarrays.
Class prediction nearest shrunken centroids DNA microarrays
2015/8/21
Class prediction by nearest shrunken centroids,with applications to DNA microarrays..