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Site-Specific Conversion of Cysteine Thiols into Thiocyanate Creates an IR Probe for Electric Fields in Proteins
food packaging perfluorinated dietary intake human exposure
2016/5/24
The nitrile stretching mode of the thiocyanate moiety is a nearly ideal probe for measuring the local electric field arising from the organized environment of the interior of a protein. Nitriles were ...
Vibrational Stark Spectroscopy in Proteins:A Probe and Calibration for Electrostatic Fields
affinity partition plasma membrane IGF-I receptor C6 glioma wheat germ agglutinin two-phase partitioning
2016/5/23
We report the first measurement of the vibrational Stark effect in a protein, providing quantitative information on the sensitivity of a vibrational transition to an applied electric field. This can b...
Insight into the sequence specificity of a probe on an Affymetrix GeneChip by titration experiments using only one oligonucleotide
microarray cross-hybridization probe length hybridization probe-level signal
2010/12/2
High-density oligonucleotide arrays are powerful tools for the analysis of genome-wide expression of genes and for genome-wide screens of genetic variation in living organisms. One of the critical pro...
Rapid Sex Identification of Chicken by Fluorescence In Situ Hybridization Using a W Chromosome-specific DNA Probe
Chicken W Chromosome XhoI family PCR FISH Sexing
2016/4/12
It has been known that the sex of chicken cells can be most accurately identified by fluorescence in situ hybridization (FISH). However, the presently available FISH has not been widely used for sex i...