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Elevated Seminal pH and Ureaplasma Urealyticum
Ureaplasma urealyticum elevated seminal pH pyosemia minocycline
2009/2/19
Normal semen has up to three polymorphonuclear leukocytes per high power field (HPF). Reduced zinc concentrations (<15 mg/dl), elevated pH (>7.7), and neutrophils (5-10 leukocytes/HPF) in semen can ...
Rat Testicular Phospholipase A2 Activity:pH Optima, Its Cellular and Subcellular Distribution in the Gonad, and Some Factors That May Modulate Its Activity
rat testicular phospholipase A2 activity pH optima localization control
2009/2/19
A sensitive radiometric assay was developed for detecting phospholipase A1 and A2 (PLA1 and A2) activity. Four to five times more PLA2 activity was observed in rat testes than PLA1 activity. Two PLA...
PH-20 is a sperm plasma-membrane protein that has been shown to have hyaluronidase activity in several mammalian species including nonhuman primates. In this investigation, the PH-20 protein was c...
Snow leopard(Panthera uncia)spermatozoa are sensitive to alkaline pH, but motility in vitro is not influenced by protein or energy supplements
Felid comparative biology sperm culture medium sperm function capacitation
2009/2/16
To better understand the biology of snow leopard spermatozoa and to facilitate developing assisted reproduction, a series of studies was conducted to: 1) identify the component(s) of complex cultu...
Gastricsin-mediated proteolytic degradation of human seminal fluid proteins at pH levels found in the human vagina
Gastricsin-mediated proteolytic degradation human seminal fluid proteins pH levels
2009/2/12
The proteolytic degradation of human seminal fluid proteins at acidic conditions has been investigated. Upon acidification to the pH level of the human vagina, autoproteolysis of most seminal fluid ...
Importance of glycosylation and disulfide bonds in hyaluronidase activity of macaque sperm surface PH-20
Enzyme activity glycoprotein protein structure deglycosylation
2008/12/30
PH-20 is a glycoprotein located on the surface of the sperm plasma membrane and on the inner acrosomal membrane. The best understood function of sperm surface PH-20 is its hyaluronidase activity, ...
Mouse Spam1(PH-20):evidence for its expression in the epididymis and for a new category of spermatogenic-expressed genes
Mouse Spam epididymis spermatogenic-expressed genes
2008/12/25
The gene for the sperm adhesion molecule 1 (PH-20), SPAM1, has been known to be testis-specific and exclusively haploid expressed. We show that in mice, the 2 common isoforms of the protein (Spam1...
Acidification of intracellular pH in bovine spermatozoa suppresses motility and extends viable life
Acidification of intracellular pH bovine spermatozoa motility viable life
2008/12/25
Intracellular pH (pHi) was determined in ejaculated bovine spermatozoa using a ratiometric absorbance technique under various incubation conditions that drastically altered sperm motility. The pHi w...
Mouse Epididymal Spam1 (PH-20) Is Released in the Luminal Fluid With its Lipid Anchor
GPI-anchor insoluble particles epididymosomes
2008/12/18
Previously we demonstrated that the murine sperm adhesion molecule 1 (Spam1 or PH-20) is synthesized by the epididymal epithelium, preferentially in the distal region, and is released into the lumin...
Changes During Epididymal Maturation, Effects of pH, Osmotic Pressure, and Knockout of the c-ros Gene
Photobleaching analysis lipid dynamics fertilizing capacity germ cells
2008/12/3
It is well known that the plasma membranes of mammalian spermatozoa undergo extensive remodeling during maturation in the epididymal duct. In this investigation, we have used fluorescence recovery a...
Importance of glycosylation and disulfide bonds in hyaluronidase activity of macaque sperm surface PH-20
Importance glycosylation disulfide bonds hyaluronidase activity macaque sperm surface PH-20
2008/12/2
PH-20 is a glycoprotein located on the surface of the sperm plasma membrane and on the inner acrosomal membrane. The best understood function of sperm surface PH-20 is its hyaluronidase activity, wh...